Pepa Whakamātautau Antibody Qrf Kore i tapahia

Ko te kitenga o te antibody wheori hipi pox he mea tito he microplate kua pania i mua ki te antigen pūmua karihi hipi pox, tohu whākōkī me ētahi atu tauhohe tautoko, a ka whakamahia te kaupapa o te enzyme-linked immunoassay (ELISA) ki te kite i te antibody pox hipi i roto i te tauira serum hipi.I te wa o te whakamatautau, ka taapirihia te serum mana me te tauira hei tirotirohia ki te pereti microplate, a, ki te mea kei roto i te tauira he antibody pox hipi i muri i te incubation, ka herea ki te antigen i runga i te pereti microplate, me etahi atu waahanga kaore i herea ka tangohia i muri i te horoinga;Kātahi ka tāpirihia te tohu whākōkī kia herea motuhake ki te matatini antigen-antibody i runga i te pereti microplate;Ko nga tohu o te enzyme kaore i herea ka tangohia ma te horoi, ka taapirihia te otinga tïpako TMB ki nga puna, ka hangaia te hua kikorangi e te tauhohenga o nga conjugates microplate, a he pai te hono o te hohonu o te tae ki te nui o nga antibodies kei roto i te tauira.I muri i te tapiritanga o te otinga whakamutu ki te whakamutu i te tauhohenga, ka kowhai te hua;Ko te uara ngongo o ia puna hohenga ka whakatauhia e te kaipanui microplate i te roangaru o te 450 nm ki te whakatau mena kei roto i te tauira he paturopi hipi.