WHAKARAPOPO ME TE WHAKAMAHI I TE WHAKAMATAU
Ko te Kolera he mate hopuhopu nui e tohuhia ana e te mate nui o nga wai o te tinana me nga electrolytes na roto i te mate korere.Ko te maaa take o te cholera kua tohua ko Vibrio cholerea (V. Cholerae), he huakita kino karamu, ka tukuna ki te tangata ma te wai poke me te kai.
Ko te momo V. Cholerae kua wehea ki etahi roopu roopu i runga i nga momo antigens O.Ko nga roopu iti O1 me O139 he mea whakahirahira na te mea ka pa mai te mate uruta me te mate kolera.He mea nui kia tere te whakatau i te noho o te V. cholerae O1 me te O139 i roto i nga tauira haumanu, te wai, me te kai kia taea ai te aro turuki tika me nga mahi aukati whai hua e nga mana whakahaere hauora.
Ko te Cholera Ag Rapid Test ka taea te whakamahi tika i roto i te mara e nga kaimahi karekau he mohio, he iti rawa ranei nga pukenga me te hua ka kitea i roto i te iti iho i te 10 min, kaore he taputapu taiwhanga uaua.
MĀTĀPONO
Ko te Cholera Ag Rapid Test he whakamaarama chromatographic immunoassay.Kei roto i te rīpene whakamātautau: 1) he papa conjugate tae burgundy kei roto he monoclonal anti-V.Ko te mate paturopi Kolera O1 me O139 i honoa ki te koura colloid (O1/O139-antibody conjugates) me te konukawata IgG-koura rapeti, 2) he riipene nitrocellulose membrane kei roto e rua nga roopu whakamatautau (1 me te 139 roopu) me te roopu whakahaere (C band).Ko te roopu 1 kua pania ki te monoclonal anti-V.Kolera O1 antibody.Ko te roopu 139 kua pania ki te monoclonal anti-V.Kolera O139 antibody.Ko te roopu C kua pania ki te koati anti-kiore IgG antibody.
Ina hoatu he rahinga o te tauira whakamatautau ki roto i te puna tauira o te rīpene whakamatautau, ka heke te tauira ma te mahi kapi puta noa i te rīpene.V. Kolera O1/O139 antigen mena kei roto i te tauira ka herea ki te whakakotahitanga koura O1/O139-antibody.Ko tenei immunocomplex ka mau ki te kiriuhi e te anti-V kua pania-mua.Ko te Kolera O1/O139 antibody, ka hangaia he roopu whakamatautau tae burgundy, e tohu ana he hua whakamatautau pai te Cholera O1/O139.Ko te kore o te roopu whakamatautau e whakaatu ana he hua kino.
Kei roto i te whakamatautau he mana o roto (C band) e whakaatu ana i te roopu tae burgundy o te immunocomplex o te koati anti-kiore IgG/kiore IgG-koura conjugate ahakoa te whanaketanga o te tae o te roopu whakamatautau.Ki te kore, he muhu te hua whakamatautau, me whakamatau ano te tauira ki tetahi atu taputapu.
