WHAKARAPOPO ME TE WHAKAMAHI I TE WHAKAMATAU
Ko te Chikungunya he mate mate onge e tukuna ana e te ngau o te namu Aedes aegypti kua pangia.Ko te ahua o te pupuhi, te kirika, me te mamae nui o nga hononga (arthralgias) e toru ki te whitu nga ra.I ahu mai te ingoa mai i te kupu Makonde ko te tikanga "ko te mea e piko ana" e tohu ana i te ahua piko i puta mai i nga tohu rewharewha o te mate.Ka puta i te wa ua
i nga waahi ngaru o te ao, ko te nuinga o Awherika, ki te tonga-rawhiti o Ahia, ki te tonga o Inia me Pakistan.Ko te nuinga o nga wa ko nga tohu karekau e kitea i roto i te haumanu i te ahua o te kirika dengue.Inaa, kua panuitia nga mate e rua o te dengue me te chikungunya i Inia.Kaore i rite ki te dengue, he onge noa te ahua o te hemorrhagic, a ko te nuinga o te wa ko te mate ko te mate kirikaa.Na reira he mea tino nui
ki te wehe i te mate dengue mai i te mate CHIK.Ka tohua te CHIK i runga i te tātaritanga serological me te wehenga viral i roto i nga kiore me te ahurea kiko.Ko te IgM immunoassay te tikanga whakamatautau taiwhanga tino whaihua.Ko te Chikungunya IgG/IgM Rapid Test e whakamahi ana i nga antigens recombinant i ahu mai i tona hanganga pūmua, ka kitea te IgG/IgM anti-CHIK i roto i te serum manawanui, plasma ranei i roto i te 20 meneti.Ka taea te whakamatautau ma te hunga kaore i whakangunguhia
he iti rawa nga kaimahi mohio, kaore he taputapu taiwhanga uaua.
MĀTĀPONO
Ko te Chikungunya IgG/IgM+NS1 antigen Tere Whakamātautau he tohu immunoassay chromatographic rere taha.Ko te rīpene whakamātautau he IgG/IgM Strip me te NS1 strip.
IgG/IgM strip:1) he papa conjugate tae burgundy kei roto i nga antigens envelope Chikungunya recombinant kua honoa ki te koura colloid (nga conjugates dengue) me te konukawata IgG-koura rapeti,2) he takai kiriuhi nitrocellulose kei roto e rua nga here whakamatautau (pae G me M) me te roopu whakahaere (C band).Ko te roopu G kua pania i mua ki te antibody mo te kitenga o te IgG anti-Chikungunya huaketo, ka pania te roopu M ki te antibody mo te kitenga o te IgM anti-Chikungunya huaketo, me te roopu C kua pania ki te koati anti rabbit IgG.
Unuhia NS1:1) he papa conjugate tae burgundy kei roto antigen anti-Chikungunya NS1 kiore i honoa ki te koura colloid (Chikungunya Ab conjugates), 2) he riipene kiri nitrocellulose kei roto he roopu whakamatautau (T band) me te roopu whakahaere (C band).Ko te roopu T kua whakakikoruatia ki te rapeti anti-Chikungunya NS1 antigen, a ko te roopu C kua pania ki te koati anti-kiore IgG antibody.
IgG/IgM Strip:I te wa e toha ana te rahinga o te tauira whakamatautau ki roto i te puna tauira o te rīpene whakamatautau, ka heke te tauira ma te mahi kapi puta noa i te rīpene.IgG anti-Chikungunya huaketo mena kei roto i te tauira ka herea ki nga hononga Chikungunya.Ka hopukina te immunocomplex e te reagent kua pania ki te roopu G, ka hanga i te roopu G tae burgundy, e tohu ana he hua whakamatautau pai IgG huaketo Chikungunya me te tohu he mate hou, he mate tuarua ranei.Ko te huaketo anti-Chikungunya IgM, mena kei roto i te tauira, ka herea ki nga hononga Chikungunya.Ka mauhia te immunocomplex e te reagent kua pania-mua ki runga i te roopu M, ka hangaia he roopu M tae burgundy, e tohu ana i te hua whakamatautau pai IgM huaketo Chikungunya me te tohu he mate hou.Ko te kore o nga roopu whakamatautau (G me te M) e tohu ana he hua kino. Kei roto i te whakamatautau he mana o roto (C band) me whakaatu he roopu tae burgundy o te immunocomplex o te koati anti rabbit IgG/
rapeti IgG-koura conjugate ahakoa te whanaketanga o te tae o tetahi o nga roopu T.Ki te kore, he muhu te hua whakamatautau, me whakamatau ano te tauira ki tetahi atu taputapu.
NS1 Wehenga: Ina tukuna he rōrahi rawaka o te tauira whakamātautau ki roto i te puna tauira o te rīpene, ka heke te tauira mā te mahi kapi puta noa i te rīpene whakamātautau.Ko Chikungunya NS1 Ag mena kei roto i te tauira ka herea ki nga hononga Chikungunya Ab.Ka mau te immunocomplex i runga i te kiriuhi e te rapeti anti-NS1 antibody, ka hangaia he roopu T tae burgundy, e tohu ana he hua whakamatautau Chikungunya Ag pai.Ko te kore o te roopu T e whakaatu ana he hua kino.Kei roto i te whakamatautau he mana o roto (C band) e whakaatu ana i te roopu tae burgundy o te immunocomplex o te koati anti-kiore IgG/kiore IgG-koura conjugate ahakoa te ahua o te roopu T tae.Ki te kore, he muhu te hua whakamatautau, me whakamatau ano te tauira ki tetahi atu taputapu.
